摘要 |
The present invention provides a method for detecting autoprocessed, secreted PCSK9, a protein involved in cholesterol homeostasis, and for effectively identifying compounds that inhibit autocleavage and secretion from cells. The disclosed method involves the insertion of an epitope tag into a PCSK9 expression construct immediately C-terminal to the pro domain ending at an amino acid residue corresponding to Q152 of human PCSK9. Upon autoprocessing, the epitope tag is exposed and capable of recognition by anti-epitope antibodies or other suitable identification system, allowing for the selective and exclusive identification and/or quantification of processed PCSK9. The present disclosure thus advances the goal of providing enabling technology to the art for the effective identification of therapeutics effective in combating coronary heart disease. |
申请人 |
MERCK & CO., INC.;SITLANI, AYESHA;FISHER, TIMOTHY, S.;SANTORO, JOSEPH, C. |
发明人 |
SITLANI, AYESHA;FISHER, TIMOTHY, S.;SANTORO, JOSEPH, C. |