| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for saccharifying a cellulose-containing material containing calcium carbonate. SOLUTION: The method for saccharifying the cellulose-containing material includes: a first step (saccharification step 1) for obtaining a sugar-containing liquid containing a formed soluble sugar by adding a cellulase (enzyme 5) containing at least an endoglucanase, an exoglucanase and aβ-glycosidase to the cellulose-containing material 4 containing the calcium carbonate, and hydrolyzing at least the cellulose in a neutral or alkaline region; a second step (solid-liquid separation step 2) for obtaining a sugar-containing liquid 7 by subjecting the sugar-containing liquid to solid-liquid separation to remove a saccharification residue 6 containing the calcium carbonate; and a third step for obtaining a saccharified liquid 9 containing a low-molecular sugar including glucose in a neutral or acidic region by adding a cellulase (enzyme 8) containing at least an endoglucanase, an exoglucanase and aβ-glycosidase. COPYRIGHT: (C)2008,JPO&INPIT
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