Method for detection of microorganism and kit for detection of microorganism

摘要

Provided is a method for detecting live cells by distinguishing live uninjured cells from dead cells and injured cells of a microorganism in a test sample, which comprises the following steps: a) the step of treating the test sample with a topoisomerase poison and/or a DNA gyrase poison, b) the step of extracting DNA from the test sample, and amplifying a target region of the extracted DNA by PCR wherein the length of the target region is 100 to 3000 nucleotides, and c) the step of analyzing an amplification product.