发明名称 |
CULTIVATION METHOD FOR STIMULATION CELL PROLIFERATION AND INHIBITION DIFFERENTIATION OF HUMAN KERATINOCYTES USING THE MEDIUM CONTAINING SOY PROTEIN HYDROLYSATES |
摘要 |
PURPOSE: A method for culturing human keratinocytes is provided to strengthen in vitro proliferation and delay differentiation with less risk. CONSTITUTION: A medium for culturing human keratinocytes in vitro contains 24ug/ml of bovine pituitary extract and 0.25-2 g/L of soybean protein hydrolysate A method for culturing the human keratinocytes comprises: a step of isolating keratinocyte from preputium from a 10-15 year old child by maintaining a skin tissue in 0.05% trypsin-0.53mM EDTA solution at 4°C for 16 hours; a step of collecting keratinocytes by shaking the skin tissue vigorously; and a step of culturing the keratinocytes in a medium containing 0.2 ng/ml of EGF(Epidermal Growth Factor), 5ug/ml of insulin, 5ug/ml of transferring, 0.18 ug/ml of hydrocortisone, 0.2%(v/v) of bovine pituitary extract(BPE).
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申请公布号 |
KR20100024305(A) |
申请公布日期 |
2010.03.05 |
申请号 |
KR20080083100 |
申请日期 |
2008.08.25 |
申请人 |
KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION |
发明人 |
CHUNG, NAM HYUN;LEE, YONG KWON;KIM, SEUNG YEUL;KIM, KI HEON;CHUN, BOK HWAN |
分类号 |
C12N5/071;C12N5/00;C12N5/04 |
主分类号 |
C12N5/071 |
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