发明名称 |
Fluorescence polarization assays for determining clostridial toxin activity |
摘要 |
<p>The present invention provides a method of determining the presence or activity of a clostridial toxin by (a) treating with a sample, under conditions suitable for clostridial toxin protease activity, a clostridial toxin substrate which includes a fluorophore; a bulking group; and a clostridial toxin recognition sequence containing a cleavage site that intervenes between the fluorophore and the bulking group; (b) exciting the fluorophore with plane polarized light; and (c) determining fluorescence polarization of the treated substrate relative to a control substrate, where a change in fluorescence polarization of the treated substrate as compared to fluorescence polarization of the control substrate is indicative of the presence or activity of the clostridial toxin.</p> |
申请公布号 |
EP2107112(A1) |
申请公布日期 |
2009.10.07 |
申请号 |
EP20090160607 |
申请日期 |
2005.09.09 |
申请人 |
ALLERGAN, INC. |
发明人 |
WILLIAMS, DUDLEY J.;GILMORE, MARCELLA A.;STEWARD, LANCE E.;VERHAGEN, MARC;AOKI, KEI ROGER |
分类号 |
C12N9/52;C12Q1/37;G01N33/48 |
主分类号 |
C12N9/52 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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