GENE INCREASING METABOLIC AVAILABILITY OF GALACTOSE, RECOMBINANT VECTOR AND RECOMBINANT MICROORGANISM CONTAINING THE SAME

摘要

PURPOSE: A gene which enhances metabolic rate of galactose is provided to enhance productivity of bioalcohol from biomass. CONSTITUTION: A gene which enhances metabolic rate of galactose is formed by losing whole or partial expression suppressing domain in a regulatory gene which suppresses expression of galactose matobolic gene. The regulatory gene is a gene encoding a TUP1 protein. A domain suppressing expression of the gene is a C-terminal repressor domain. The regulatory gene has a polynucleotide sequence of sequence number 1. The TUP1 protein has an amino acid sequence of sequence number 2. A pRS424 recombinant vector contains the of sequence number 1. A transformed recombinant microorganism is prepared using the recombinant vector. The microorganism is yeast. The recombinant microorganism is Saccharomyces cerevisiae CEN.PK2-1D/pRS424-truncated TUP1 of deposit number KCTC 11387 BP.