OLIGONUCLEOTIDES FOR DETECTION OF PLANT-INFECTING BACTERIAL OR FUNGAL SPECIES

摘要

PURPOSE: An oligonucleotide for detecting plant pathogenic bacteria and fungi is provided to simultaneously amplify a target sequence of bacteria and fungi and to ensure excellent workability in multiplex PCR. CONSTITUTION: An oligonucleotide which is hybridized with plant pathogenic bacteria or fungi is denoted by formula 5'-Xp-Yq-Zr-3'. In the formula, Xp is a 5'-first priming portion having complementary hybridization sequence with a target sequence; Yq is a separation portion containing at least two or more universal bases; Zr is a 3'-second priming portion having complementary hybridization sequence with the target sequence; P, q, and R is the number of nucleotide; and X, Y, and Z is deoxyribonucleotide or ribonuelcotide. The Tm of the 5'-first priming portion is higher than Tm of 3'-second priming portion.