METHOD FOR PRODUCTION OF PANCREATIC RIBONUCLEASE

摘要

FIELD: biotechnologies. ^ SUBSTANCE: method provides for grinding of pancreas. Ground pancreas is treated with solution of sodium chloride of 4.5-6 wt % in the following volume ratio between pancreas and salt solution equal to 1:(1.5-2.0) at the temperature of 25-35C for 2.5-3.5 hours. Produced cells of pancreas are separated by centrifugation and treated with 95% ethyl alcohol for 15-24 hours in volume ratio of 1:(0.85-1.3) at the temperature of 15-25C. Pancreas cells are separated by centrifugation with further treatment of these cells with water acidified with hydrochloric acid to pH of 1.0-3.0 for 2.5-4.5 hours at the temperature of 25-35C in volume ratio of 1:(0.8:1.2). Produced cells of pancreas are separated by centrifugation to produce supernatant. Ribonuclease is extracted from produced supernatant by solutions of sulfuric acid with concentration of 0.15-0.35 for 3-4 hours with volume ratio of 1:(0.8-1.2) at the temperature of 4-6C. Suspended particles are separated by centrifugation to produce extract that contains ribonuclease. Produced extract is concentrated by ultrafiltration on UPM 10 membrane with further diafiltration. Produced concentrate is cooled down to 4-6C, and ribonuclease is deposited at pH of 8.0-9.0. Produced residue is washed with ethyl alcohol in volume ratio of 1:5 and dried. ^ EFFECT: increased yield of ferment. ^ 1 tbl, 1 ex