IMMUNOGLOBULIN COMPOSITIONS AND METHODS OF PRODUCING SAME

摘要

A method of producing an immunoglobulin in a bacterial culture, the method comprising: (a) expressing in a first bacterial host cell a first polypeptide comprising an immunoglobulin light chain so as to form inclusion bodies which comprise the immunoglobulin light chain; (b) expressing in a second bacterial host cell a second polypeptide comprising an immunoglobulin heavy chain so as to form inclusion bodies which comprise the immunoglobulin heavy chain; (c) recovering from the inclusion bodies the first polypeptide and the second polypeptide so as to obtain reconstituted heavy chain and reconstituted light chain; and (d) refolding the reconstituted heavy chain and reconstituted light chain under conditions which allow refolding of the reconstituted light chain and the reconstituted heavy chain predominantly as an intact immunoglobulin.