| 摘要 |
Apparatus for carrying out real-time PCR reactions, including a thermocycler having a reaction region with a plurality of temperature-regulable receptacles for reaction vessels, an illumination device, which has a plurality of light-emitting diodes and is assigned to the reaction region and by means of which excitation light can be radiated into the receptacles, a detector device, which generates measured values in a manner dependent on a measured light intensity, optical devices defining a beam path that leads from the illumination device to the receptacles and from there to the detector device, a reference device, which generates a reference measured value by measurement of the light intensity of a light-emitting diode, and an evaluation device, which takes into account the reference measured value with the measured values, wherein the reference device has a reference light-emitting diode, the light of which is coupled into the beam path behind the reaction region. |
