发明名称 |
ACTIVATED SPLIT-POLYPEPTIDES AND METHODS FOR THEIR PRODUCTION AND USE |
摘要 |
The present invention relates to a method to produce activated split-polypeptide fragments that on reconstitution immediately forms an active protein. The method relate to real-time protein complementation. Also encompassed in the invention is a method to split and produce split-fluorescent proteins in an active state which produce a fluorescent signal immediately on reconstitution. The present application also provides methods to detect nucleic acids; non-nucleic acid analytes and nucleic acid hybridization in real-time using the novel activated split-polypeptide fragments of the invention. |
申请公布号 |
US2009220942(A1) |
申请公布日期 |
2009.09.03 |
申请号 |
US20060091709 |
申请日期 |
2006.10.27 |
申请人 |
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发明人 |
BROUDE NATALIA;CANTOR CHARLES R.;DEMIDOV VADIM V. |
分类号 |
C12Q1/70;C07K14/00;C12P21/00;C12Q1/68 |
主分类号 |
C12Q1/70 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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