| 摘要 |
FIELD: medicine. ^ SUBSTANCE: method for preparing samples of non-decalcified articular cartilage with subjacent subchondral bone for multipurpose study involves tissue preparation for transmission electronic microscopy. Tissue samples 3-4 mm are taken and dehydrated in the mode of smooth alcohol to acetone transition with time increase at the stages from 70% to 96% of alcohol per 1 h. Then they are dehydrated in 2 changes 70% of alcohol for 1 h, in 2 changes 80% of alcohol for 1 h, in 2 changes 90% of alcohol for 1 h, in 2 changes 96% of alcohol for 1 h, in 2 changes 100% of alcohol for 30 min, in 2 changes 100% of acetone for 30 min. It is followed by impregnation in the mode of smooth alcohol to acetone transition: resin-acetone composition 100% 3:1 for 2 h, resin-acetone composition 100% 1:1 for 2 h, resin-acetone composition 100% 1:3 for 2 h. Before polymerisation, the sampled are impregnated by resin at room temperature to 10 days. ^ EFFECT: provided qualitative and quantitative study with the use of microscopy techniques. ^ 3 cl, 6 dwg |
