| 摘要 |
PROBLEM TO BE SOLVED: To provide: an siRNA having a high gene-inhibiting effect, which is double-stranded RNA having target gene expression inhibiting ability and 21 to 27 bases and is used in RNA interference methods; and a method for efficiently inhibiting gene expression using the siRNA. SOLUTION: There are disclosed: an siRNA preparing method in which a low molecular weight compound having an unsaturated hydrocarbon compound as a basic skeleton is conveniently, efficiently and chemically modified to ribonucleotide by known methods such as a phosphoroamidite method or an active esterification method using a succinimide linker; and a method for efficiently inhibiting gene expression that is represented by an RNA interference method using the siRNA. COPYRIGHT: (C)2011,JPO&INPIT
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