摘要 |
The invention relates to a method of preparing a variant of a parent polypeptide comprising: (a) providing an amino acid sequence of a parent polypeptide; (b) substituting at least one amino acid residue at a position in the sequence corresponding to any of positions: 41, 83, 129, 207 or 284 in SEQ ID No: 2; (c) selecting a variant with lipolytic activity, which compared to the parent polypeptide has improved stability, and has an amino acid sequence with at least 60% identity to the mature polypeptide of SEQ ID No: 2; and (d) recovering the variant. |
申请人 |
NOVOZYMES A/S;NOVOZYMES SOUTH ASIA PVT. LTD.;SVENDSEN, ALLAN;NAIK, SANGEETA;SAIKIA, RAKHI;BASU, ADITYA;PAUL, PRITISH;VASU, SANTHOSH, MAYPADUM;DE MARIA, LEONARDO;SKJOET, MICHAEL |
发明人 |
SVENDSEN, ALLAN;NAIK, SANGEETA;SAIKIA, RAKHI;BASU, ADITYA;PAUL, PRITISH;VASU, SANTHOSH, MAYPADUM;DE MARIA, LEONARDO;SKJOET, MICHAEL |