METHOD FOR CONSTRUCTING CHIMERIC RAT USING RAT EMBRYONIC STEM CELLS

摘要

Provided is a method for constructing a chimeric embryo and a chimeric rat, characterized by comprising contacting rat pluripotent stem cells with a host embryo in the presence of an ES cell differentiation inhibitor. This method comprises a step (a) for contacting a host embryo, which is collected from a female rat after fertilization, with rat pluripotent stem cells in the presence of an ES cell differentiation inhibitor; and a step (b) for incubating the host embryo having been contacted with the rat pluripotent stem cells to form a chimeric embryo.