摘要 |
Plant viral vectors have great potential in rapid production of proteins, but no simple. Here a geminivirus-based system for high-yield and rapid production of oligomeric protein complexes, including virus-like particle (VLP) vaccines and monoclonal antibodies (mAbs) is described. In particular, a single vector that contains two non-competing replicons for transient expression in Nicotiana benthamiana leaves is described. The correct assembly of these subunit proteins into functional oligomeric structures (VLPs or full-size mAb) is also described. This system advances plant transient expression technology by eliminating the need for non-competing viruses, and thus, enhances the realistic commercial application of this technology for producing multiple-subunit protein complexes. |
申请人 |
ARIZONA BOARD OF REGENTS FOR AND ON BEHALF ARIZONA STATE UNIVERSITY |
发明人 |
MASON, HUGH, S.;HUANG, ZHONG;CHEN, QIANG;ARNTZEN, CHARLES, J.;YUAN, SHUO;HJELM, BROOKE |