RECOMBINANT PROTEIN Collbd-BMP-2, RECOMBINANT PLASMID pCollbd-BMP-2, STRAIN Escherichia coli-PRODUCER OF RECOMBINANT PROTEIN Collbd-BMP-2, METHOD TO PRODUCE RECOMBINANT PROTEIN Collbd-BMP-2

摘要

FIELD: medicine. ^ SUBSTANCE: recombinant protein Collbd-BMP-2 is described, consisting of collagen-binding domain Collbd (SPARC(w)), of extracellular calcium-dependent protein SPARC (VM-40 or osteonectin) of a human being and spacer sequence GGS. Method for its production is proposed, including growing of strain E. coli M15 [pREP4, pCollbd-BMP-2]. According strain and plasmid are proposed. ^ EFFECT: invention makes it possible to provide a high level of recombinant protein Collbd-BMP-2 production and simultaneously simple and efficient scheme for cleaning of this protein with its immobilisation on collagen-containing sorbent included into composition of new generation of items and materials of medical purpose - implants and coatings. ^ 4 cl, 1 dwg, 3 ex