发明名称 |
PROBE, KIT, AND METHOD OF AMPLIFICATION FOR INCREASING THE SENSITIVITY OF NUCLEIC ACID HYBRIDIZATION ASSAYS |
摘要 |
The present invention provides a method for simplifying and significantly enhancing the sensitivity of nucleic acid hybridization assays. A method is described whereby a single-stranded primary nucleic acid sequence that includes a region of sequences complementary to a single-stranded target nucleic acid sequence is hybridized to the target molecule. Stability of the double-stranded complex thereby formed can be enhanced by using RNA as the probe if DNA is the target or DNA as the probe if RNA is the target. The probe-target complex is subsequently immunocaptured for detection. After washing away- extraneous material, a secondary nucleic acid sequence containing many repeating sequence units is hybridized to the probe component of the immobilized probe-target complex. Detection occurs following hybridization of many labelled nucleic acid sequence probes to each of the repeating sequence units of a nucleic acid amplification probe. Thus, attachment of multiple labelling probes to an amplification probe that is hybridized to an immobilized probe-target complex, provides a simplified method for amplifying the detection signal and therefore the sensitivity of nucleic acid hybridization assays.
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申请公布号 |
CA2126952(C) |
申请公布日期 |
2010.12.14 |
申请号 |
CA19942126952 |
申请日期 |
1994.06.28 |
申请人 |
KALYX BIOSCIENCES INC. |
发明人 |
PANDIAN, SITHIAN;AW, ENG JOM;SMITH, DAVID I. |
分类号 |
C12Q1/70;G01N33/58;C07H21/00;C12N15/09;C12Q1/68 |
主分类号 |
C12Q1/70 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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