摘要 |
FIELD: medicine. ^ SUBSTANCE: four identical suspensions of test microorganisms are produced to estimate activity of an antimicrobial preparation. The first one contains physiologic saline, the second one - the antimicrobial preparation in the preset concentration Cprep, the third one - physiologic saline, and the fourth one - the antimicrobial preparation in the same amount. After a certain period of time td, a detergent is added to the third and fourth suspensions, while two first samples of suspension are filled with physiologic saline so that microorganism concentration are the same in all four samples of suspension. The fluorescence levels of these suspensions are measured with a laser and/or non-laser fluorometre at the moment t1 and after an interval öt equal to 15 minutes approximately. Then measurements are performed once again at the moment t2 and after an interval öt. Further, the measured values are used to calculate the antimicrobial preparation efficacy by solving kinetic equations. ^ EFFECT: use of the method allows cutting time of estimated efficien of the antimicrobial preparation, more precise and simplified procedure of choosing a preferential antimicrobial preparation. ^ 2 cl, 1 tbl, 1 ex |