| 摘要 |
The present invention provides genes encoding novel luciferases having at least the properties of: being capable of using coelenterazine as their luminescent substrates; and being capable of being recombinantly expressed in a mammal cell as a host and produced to be secreted to the outside of the host cell. Specifically, the gene encoding novel luciferases according to the present invention is a DNA molecule comprising a nucleotide sequence encoding any of the full-length amino acid sequences of two types of luciferase proteins, luciferase 1 and luciferase 2, from M. pacifica, and is, for example, a gene encoding the following full-length amino acid sequence of the luciferase 1. MMEIQVLFAL ICFALVQANP TENKDDIDIV GVEGKFGTTD 60 LETDLFTIVE DMNVISRDTN LANSDADRGK MPGKKLPLEV LIEMEANARK AGCTRGCLIC 120 LSKIKCTAKM KVYIPGRCHD YGGDKKTGQA GIVGAIVDIP EISGFKELGP MEQFIAQVDL 180 CADCTTGCLK GLANVKCSAL LKKWLPDRCA SFADKIQSEV DNIKGLAGDR 210
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