Producing adherent cells comprises introducing adherent cell suspension into culture vessel containing microcarriers in culture medium, amplifying cells by performing cell passages, and harvesting cells produced during the last cell passage

摘要

Producing adherent cells comprises: (a) introducing a suspension of adherent cells into a culture vessel containing microcarriers in a culture medium; (b) amplifying the cells by performing many cell passages in the same culture vessel; and (c) harvesting the cells produced during the last cell passage, optionally after having subjected the cells to enzyme treatment to detach cells from microcarriers. Producing adherent cells comprises: (a) introducing a suspension of adherent cells into a culture vessel containing microcarriers in a culture medium; (b) amplifying the cells by performing many cell passages in the same culture vessel, where each cell passage subsequent to the first cell passage is carried out by using at least part of the cells produced during the previous cell passage after having subjected the cells to enzyme treatment to detach the cells from microcarriers, and introducing culture medium and an increasing amount of microcarriers; and (c) harvesting the cells produced during the last cell passage, optionally after having subjected the cells to enzyme treatment to detach cells from microcarriers. Independent claims are also included for: (1) producing adherent cells comprising thawing a stock of adherent cells, and then subjecting the thawed adherent cells to the process mentioned above; and (2) producing a biological agent from adherent cells comprising: steps (a) and (b) as mentioned above; (c1) treating the cells produced during the last cell passage such that it produces the biological agent, where the treating is carried out in the same culture vessel as the one used to amplify the cells; and (d) harvesting the biological agent.