| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method for culturing human embryonic stem cells without the need for feeder cells or for exposure of the medium to feeder cells. <P>SOLUTION: The stem cells are cultured, in an environment essentially free of mammalian fetal serum and in a stem cell culture medium including amino acids, vitamins, salts, minerals, transferrin, insulin, albumin, and a fibroblast growth factor that is supplied from a source other than a feeder layer medium. <P>COPYRIGHT: (C)2012,JPO&INPIT |
