| 摘要 |
The invention provides a method for detecting nucleotide mutation sites. The method comprises the following steps of: (1) determining the position of a mutation site in a to-be-detected sequence; (2) designing a primer according to the position of the position of the mutation site; (3) performing PCR amplification; (4) performing SAP enzyme treatment so as to remove dNTPs contained by a amplification product; (5) performing extension reaction and connecting the 3' end of an extension product with a basic group; (6) adopting resin to purify the extension reaction product; and (7) co-crystallizing the purified product and a chip matrix, putting a crystal into a vacuum tube of a mass spectrograph and then using instantaneous nanosecond intense laser excitation to perform mass spectrometric detection so as to determine the genotype of mutation. The detection method solves the problem that the prior method for detecting nucleotide mutation sites is low in sensitivity and accuracy. |
