| 摘要 |
PROBLEM TO BE SOLVED: To provide cell culture processes.SOLUTION: The processes include culturing heterologous protein-secreting mammalian cells, such as CHO or BHK cells, at 35.1-36.5°C and/or at a pH of 7.15-7.20 and/or at a dissolved COconcentration of 10% or lower. Preferred heterologous proteins are Factor VIII, ADAMTS-13, furin or Factor VII. Other embodiments of the present invention provide a method of continuous culture of FVIII-secreting mammalian cells in a vessel comprising a cell culture supernatant; where the density of the cells in the cell culture supernatant is measured by an in-line sensor and the influx of fresh medium into the vessel is automatically controlled so as to maintain the density of the cells in a desired range. |
