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1. A method of determining the sequence of a target nucleic acid molecule, comprising:
contacting an RNA polymerase with a target nucleic acid molecule under sequencing conditions, wherein sequencing conditions comprise the presence of at least one nucleoside triphosphate, wherein said RNA polymerase is immobilized on a solid substrate, wherein the target nucleic acid molecule comprises a rotation tag; detecting the rotational pattern of the rotation tag; repeating the contacting and detecting steps a plurality of times; and determining the sequence of the target nucleic acid molecule based, sequentially, on the presence or absence of a change in the rotational pattern in the presence of the at least one nucleoside triphosphate.
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