Creating and viewing three dimensional virtual slides

摘要

Systems and methods for creating and viewing three dimensional digital slides are provided. One or more microscope slides are positioned in an image acquisition device that scans the specimens on the slides and makes two dimensional images at a medium or high resolution. These two dimensional digital slide images are provided to an image viewing workstation where they are viewed by an operator who pans and zooms the two dimensional image and selects an area of interest for scanning at multiple depth levels (Z-planes). The image acquisition device receives a set of parameters for the multiple depth level scan, including a location and a depth. The image acquisition device then scans the specimen at the location in a series of Z-plane images, where each Z-plane image corresponds to a depth level portion of the specimen within the depth parameter.

主权项

1. A method for providing digital images, the method comprising using one or more hardware processors to:
provide a base digital image; receive a selection of a first area of interest in the base digital image; acquire a first Z-stack of the first area of interest, wherein the first Z-stack comprises a first plurality of Z-planes, and wherein each of the first plurality of Z-planes comprises a digital image of the first area of interest at a different focus depth; and provide the first area of interest at one or more focus depths using the first Z-stack.