COMPOSITIONS AND METHODS FOR SAMPLE PROCESSING

摘要

This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.

主权项

1. A composition, comprising a bead that is covalently linked to a plurality of oligonucleotides comprising an identical barcode sequence and a variable domain.