| 摘要 |
The invention relates generally to methods and compositions for maintaining and manipulating microbial cultures of Gram-positive bacteria. Also provided are methods for identifying quorum sensing regulatory proteins and auto-inducing peptides in Gram-positive bacteria. Also provided are methods and compositions believed to affect quorum sensing pathways of the genus Clostridium to direct or maintain enhanced butanol production of Clostridium in a desired differentiated state during sequential or continuous culture. Differentiated states include extended serial propagation, and continuous culture, for the production of butanol or other fermentation products. Further provided are methods where the concentration of butanol in peptide treated cultures of the genus Clostridium increase more rapidly and produce a substantially greater amount of butanol than in Clostridium cultures not treated with the peptide. |
