发明名称 |
PREPARATION OF MOLECULAR IMPRINTED POLYMERS BY CROSS-LINKING |
摘要 |
Provided is an improved method for preparation of insoluble molecular imprinted polymers (MIPs), the method comprising: a) providing soluble or semi-soluble MIPs that 1) substantially all bind template agents and 2) have sizes which enable their separation in a chromatographic step utilizing packed bed chromatography, b) cross-linking the template agent binding soluble MIPs provided in step a so as to obtain insoluble template agent binding MIPs, and c) optionally isolating, concentrating or purifying the MIPs obtained by the cross-linking in step b. In an interesting embodiment, step a includes an affinity purification procedure, which ensures that the MIPs provided in step a are indeed all binders of the template. |
申请公布号 |
US2015031779(A1) |
申请公布日期 |
2015.01.29 |
申请号 |
US201214379913 |
申请日期 |
2012.02.28 |
申请人 |
Gregorius Klaus;Nicholls Ian Alan;Krogh Nicolas Otto |
发明人 |
Gregorius Klaus;Nicholls Ian Alan;Krogh Nicolas Otto |
分类号 |
C08J9/36 |
主分类号 |
C08J9/36 |
代理机构 |
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代理人 |
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主权项 |
1. A method for preparation of insoluble molecular imprinted polymers (MIPs), the method comprising:
a. providing soluble or semi-soluble MIPs that 1) substantially all bind template agents and 2) have sizes which enable their separation in a chromatographic step utilizing packed bed chromatography, b. cross-linking the template agent binding soluble MIPs provided in step a so as to obtain insoluble template agent binding MIPs, and c. optionally isolating, concentrating or purifying the MIPs obtained by the cross-linking in step b. |
地址 |
Soborg DK |