| 发明名称 |
DITERPENE PRODUCTION |
| 摘要 |
The present invention relates to a recombinant microorganism comprising one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity, whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least steviol. The recombinant microorganism may also be capable of expressing one or more UDP-glucosyltransferases such that the microorganism is capable of producing one or more steviol glycosides. |
| 申请公布号 |
US2015031868(A1) |
申请公布日期 |
2015.01.29 |
| 申请号 |
US201314373571 |
申请日期 |
2013.01.23 |
| 申请人 |
DSM IP ASSETS B.V. |
发明人 |
Lehmann Martin;Trueheart Joshua;Zwart Jens Priscilla;Wu Liang;Boer Viktor Marius;Sagt Cornelis Maria Jacobus;Kumar Manoj;Meijrink Bernard;Van Den Berg Marco Alexander |
| 分类号 |
C12P19/56;C12N9/10;A23L1/236;C07H15/256 |
主分类号 |
C12P19/56 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A recombinant microorganism comprising at least one nucleotide sequence encoding:
a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity, whereby expression of the nucleotide sequence confers on the microorganism an ability to produce at least steviol. |
| 地址 |
Heerlen NL |
