Methods for Enhancing Bacterial Cell Display of Proteins and Peptides

摘要

Methods of making and using bacterial display polypeptide libraries using circularly permuted OmpX (CPX) variants are disclosed. The invention further relates to methods for enhancing the display of proteins and peptides at the surface of bacteria by optimizing linkers and incorporating mutations at positions 165 and 166 of CPX.

主权项

1. A circularly permuted OmpX (CPX) variant comprising a linker joining the native N-terminus and native C-terminus of OmpX, wherein the CPX variant comprises a non-native N-terminus and a non-native C-terminus, and wherein the linker is 3-8 residues in length and comprises a glycine and one or more basic amino acids.