| 摘要 |
The invention pertains to the use of (i) one or more C2-C6 alpha-hydroxy acids, salts of these acids, esters of these acids and combinations thereof; and (ii) one or more compounds selected from—the group of amino acid derivatives consisting of γ-glutamyl amino acids, pyroglutamyl amino acids, glutamate-containing or proline-containing dipeptides, oxo-aminoacids, homo-amino acids, N-acetyl amino-acids and glycyl-glycine; -phenolic acid derivatives; -linear C2-C6 or cyclic C6 sugar alcohols; -pyridinic acid derivatives; and -nucleobases and/or nucleotides chosen from uracil, adenine, adenosine 3′-monophosphate (3′-AMP) and adenosine 5′-monophosphate (AMP), as a growth-and production promoting ingredient, in culture media for culturing eukaryotic cells. The invention further pertains to culture media containing these components at levels of at least 0.001 mg/l. |
| 主权项 |
1. A process of producing a culture medium for culturing eukaryotic cells comprising adding to conventional culture medium the following ingredients:
(i) one or more C2-C6 alpha-hydroxy acids, salts of these acids, esters of these acids and combinations thereof; and (ii) one or more compounds selected from the following groups of compounds:
a) amino acid derivatives consisting of γ-glutamyl amino acids, pyroglutamyl amino acids, glutamate-containing or proline-containing dipeptides, oxo-aminoacids, homo-amino acids, N-acetyl amino-acids and glycyl-glycine;b) phenolic acid derivatives;c) linear C2-C6 or cyclic C6 sugar alcohols;d) pyridinic acid derivatives; ande) nucleobases and/or nucleotides chosen from uracil, adenine, adenosine 3′-monophosphate (3′-AMP) and adenosine 5′-monophosphate (AMP), or a combination of compounds selected from compound groups a), b), c), d) and e), as a growth-promoting and/or production-improving ingredient, such that the final concentration in the culture medium is at least 0.001 mg/l per individual compound listed under (i) and (ii), wherein said compounds are added as a pure substance or as a concentrate. |
