CELL CULTURING METHOD, CELL CULTURING APPARATUS AND KIT

摘要

The present invention relates to a cell culturing method as well as a cell culturing apparatus and kit for use in said culturing method. This cell culturing method comprises applying cells on a porous polyimide film and culturing. One embodiment of the method according to the present invention comprises a process for sowing cells on the surface of the porous polyimide film. An embodiment comprises a process for placing a cell suspension on the dried surface of the porous polyimide film and leaving the porous polyimide film undisturbed or moving said porous polyimide film to promote liquid efflux or stimulating a portion of the surface to cause the cell suspension to be drawn into the film and then retaining the cells in the cell suspension inside the film while allowing the moisture to flow out. Another embodiment comprises a process for moistening one or both surfaces of the porous polyimide film with a cell culture solution or sterilized liquid, loading a cell suspension on the moistened porous polyimide film, then retaining the cells in the cell suspension inside the film, and allowing the moisture to flow out.