DETECTING ANALYTES WITH A PH METER

摘要

Provided herein are sensors, kits that include such sensors, and methods for making and using such sensors. The sensors permit detection of a broad array of target molecules, such as nucleic acids (e.g., DNA and RNA), proteins, toxins, pathogens, cells, and metals, and can be used in combination with pH meters and pH paper. Thus, this disclosure provides a new methodology that allows pH meters and pH paper to be used for the detection of analytes other than pH.

主权项

1. A method for detecting a target, comprising
contacting a sample with (a) a recognition molecule specific for the target and (b) a solid support comprising glucose oxidase, under conditions sufficient to allow the target in the sample to bind to the recognition molecule and to release the glucose oxidase from the solid support; separating the solid support from the released glucose oxidase; contacting the released glucose oxidase with glucose, thereby generating gluconic acid; and detecting a change in pH, wherein detection of a significant decrease in pH indicates the presence of the target agent in the sample, and an absence of detected significant decrease in pH indicates the absence of the target agent in the sample.