Method for calibrating variable wavelength liquid chromatography detectors

摘要

A method for determining the wavelength accuracy of a variable wavelength liquid chromatography detector in the low UV range comprising filling the cell of the detector with a test solution and varying the indicated wavelength of detection to find the indicated wavelength of maximum absorbance in a wavelength region around a reference wavelength of the test solution and then calculating the difference between the indicated wavelength of maximum absorbance and the reference wavelength to determine the accuracy of the indicated wavelength of the detector. The test solution contains terbium III ions in a solvent such as n-propanol so that the absorbance maximum of the test solution does not shift more than about 0.5 nm for a spectral bandpass ranging from 2 to 6 nm.