MULTIPLEXED DIAGNOSIS METHOD FOR CLASSICAL HODGKIN LYMPHOMA

摘要

A method for providing a composite image of a single biological sample from a patient suspected of having classical Hodgkin lymphoma, comprising the steps of generating a first image of the biological sample including the presence, absence and/or expression level of a first biomarker, generating a second image of the biological sample including the presence, absence and/or expression level of a second biomarker, and generating a composite image that provides the relative location or expression of both biomarkers. Also provided is a method of analyzing a biological sample, comprising providing a composite image of the biological sample according to the method for providing a composite image, and analyzing the expression of the biomarkers of interest from the composite image. Further provided are method for diagnosing classical Hodgkin lymphoma, as well as system and kit that comprise the means for executing the novel methods.

主权项

1. A method for providing a composite image of a single tissue sample from a patient suspected of having classical Hodgkin lymphoma which comprises:
(1) generating a first series of images of the biological sample, which step comprises:
(a) contacting the sample on a solid support with a first binder for a first biomarker;(b) staining the sample with a fluorescent marker that provides morphological information;(c) detecting, by fluorescence, signals from the first binder and the fluorescent marker; and(d) generating the first images of at least part of the sample from the detected fluorescent signals; (2) after signal removal from the first binder, generating one or more second series of images of the biological sample, which step comprises;
(a) contacting the same sample with a binder for another biomarker;(b) optionally staining the sample with a fluorescent marker that provides morphological information;(c) detecting, by fluorescence, signals from the binder and the fluorescent marker; and(d) generating the second images of at least part of the sample from the detected fluorescent signals; and (3) generating a composite image that provides the relative location or expression of both the first biomarker and the other biomarker.