NOVEL RECOMBINANT PLASMID PGRF44-22

摘要

NEW MATERIAL:A plasmid pGRF44-22, stably replicative in Escherichia coli and capable of imparting trimethoprim and ampicillin tolerance to host Escherichia coli and encoding a fused protein of a derivative of a bovine growth hormone releasing factor having an amino acid sequence expressed by the formula to a dihydrofolate reductase. USE:For producing a bovine growth hormone releasing factor(GRF) and dihydrofolate reductase(DHFR). PREPARATION:For example, a DNA fragment prepared by cleaving a plasmid pMEK2 for producing leucine enkephalin with a restriction enzyme is linked to a DNA fragment obtained by cleaving a recombinant plasmid pGRF2-15 capable of coding DHFR and an amino acid sequence of the 1st to 29th residues of a bovine GRF using a T4-DNA ligase, inserted into Escherichia coli and cultured to select the resultant colony. Thereby, the objective pGRF44-22 is obtained.