PHENOTYPE-NEUTRAL BARCODES FOR DIGITAL ANALYSIS

摘要

Processes of specifically and effectively labeling an organism are provided. Processes involve the incorporation of a plurality of phenotype neutral tags that are differentially detected where the presence or absence of the tag is represented by a digital readout. The incorporation of stealth tags or insertion tags provides a rapid and population maintaining labeling of an organism that can be readily identified by digital PCR techniques.

主权项

1. A process of labeling an organism comprising:
selecting a labeling region within an exon of a gene; substituting a plurality of non-wild type nucleotides within said labeling region to produce a plurality of tags within said labeling region, said plurality of tags representing a barcode, at least two of said plurality of tags each in a separate codon resulting in synonymous substitutions at each nucleotide substitution site; said step of substituting producing silent mutations in said gene such that a protein encoded by said gene is the wild-type amino acid sequence.