发明名称 |
CYTOKININ-ACTIVATED MACROPHAGE |
摘要 |
<p>PURPOSE:To obtain the subject macrophage having a high cell-injuring activity against a wide range of tumor cells by isolating monocytes from a human peripheral blood and subsequently culturing the monocytes in the presence of interleukin-2 and a specific cytokinin in a human serum-containing nutritive medium. CONSTITUTION:A human peripheral blood is centrifuged to remove erythrocytes, and the obtained buffy coat is treated with a percol solution to isolate highly pure monocytes. The monocytes are cultured in the presence of interleukin-2 and a cytokinin (e.g. colony-stimulating factor-1) relating to the differentiation or activation of monocyte/macrophage in a human serum-containing nutritive medium at 37 deg.C in 5% carbon dioxide atmosphere for 6-17 days, and the cytokinin-activated macrophage produced in the cultured solution is washed with a phosphoric acid buffer solution and subsequently mixed with a 2mM ethylenediamine tetraacetic acid buffer solution. The floated cells are recovered to give the objective cytokinin-activated macrophages.</p> |
申请公布号 |
JPH05130863(A) |
申请公布日期 |
1993.05.28 |
申请号 |
JP19910296567 |
申请日期 |
1991.10.17 |
申请人 |
JAPAN TOBACCO INC |
发明人 |
NISHIMURA KEIKO;AZUMA NOBUAKI;OSAWA TOSHIAKI |
分类号 |
A61K35/12;A61K45/00;C12N5/07;C12N5/0786;C12N5/09 |
主分类号 |
A61K35/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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