| 摘要 |
<p>The invention relates to a process for assembling a polynucleotide from a plurality of oligonucleotides comprising: (a) synthesizing or spotting a plurality of different oligonucleotides on a microarray device or bead device having a solid or porous surface, wherein the plurality of oligonucleotides are attached to the solid or porous surface, and wherein each oligonucleotide comprises a sequence region of from about 10 to about 50 bases that is the same or substantially the same as a sequence region of another oligonucleotide; (b) forming an oligonucleotide complementary to a first oligonucleotide by extending a first primer, wherein said primer is complementary to a portion of said first oligonucleotide; (c) is associating said complementary oligonucleotide from said first oligonucleotide and annealing said complementary oligonucleotide to another oligonucleotide comprising a sequence region that is the same or substantially the same as a sequence region of said first oligonucleotide; (d) repeating the primer extension cycles of step (c) to produce a full-length complementary polynucleotide; and (e) amplifying the assembled full length complementary polynucleotide to produce a full length polynucleotide in desired quantities.</p> |
