Materials and methods useful for affecting tumor cell growth, migration and invasion

摘要

It is disclosed herein that miR-221 and miR-222 down-regulate PTEN and TIMP3 tumor suppressors, resulting in TRAIL resistance. The present invention provides research, diagnostic, and therapeutic tools and methods related to this discovery. Diagnostics, prognostics and treatments for human hepatocellular cancer and non-small cell lung carcinoma having a TRAIL resistance are particularly described herein.

主权项

1. A method to alter regulation of TIMP3 expression in a cell capable of expressing TIMP3 and miR-221 and miR-222, comprising contacting the cell with inhibitors of miR-221 and miR-222 in an amount sufficient to increase expression of TIMP in the cell,
wherein the cell is selected from the group consisting of a non-small cell lung carcinoma cell, a hepatocellular carcinoma cell, and a melanoma cell.