Recombinant DNA constructs and methods for controlling gene expression

摘要

The present invention provides molecular constructs and methods for use thereof, including constructs including heterologous miRNA recognition sites, constructs for gene suppression including a gene suppression element embedded within an intron flanked on one or on both sides by non-protein-coding sequence, constructs containing engineered miRNA or miRNA precursors, and constructs for suppression of production of mature microRNA in a cell. Also provided are transgenic plant cells, plants, and seeds containing such constructs, and methods for their use. The invention further provides transgenic plant cells, plants, and seeds containing recombinant DNA for the ligand-controlled expression of a target sequence, which may be endogenous or exogenous. Also disclosed are novel miRNAs and miRNA precursors from crop plants including maize and soy.

主权项

1. A recombinant DNA construct comprising a promoter functional in a plant cell and operably linked to DNA that transcribes to RNA comprising:
(a) at least one exogenous miRNA recognition site recognizable by a mature miRNA, wherein said mature miRNA is encoded by a DNA sequence selected from SEQ ID NOs:36, 40, 41, or 42 and is expressed in a specific cell of a plant, and (b) target RNA to be suppressed in said specific cell, wherein said target RNA is to be expressed in cells of said plant other than said specific cell.