| 发明名称 | Modified ethylenediamine-N,N′-disuccinate:ethylenediamine lyase | ||
| 摘要 | The present invention provides a modified ethylenediamine-N,N′-disuccinate:ethylenediamine lyase. The present invention also provides a protein that comprises the amino acid sequence represented by SEQ ID NO: 1; or a protein that comprises an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 1 by deletion, substitution, or addition of one or more amino acid residues, and has an ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity. | ||
| 申请公布号 | US9212356(B2) | 申请公布日期 | 2015.12.15 |
| 申请号 | US201414243743 | 申请日期 | 2014.04.02 |
| 申请人 | MITSUBISHI RAYON CO., LTD. | 发明人 | Akiyama Takanori;Mizunashi Wataru;Nakamura Tetsuji |
| 分类号 | C12N9/88;C12P13/00;C07H21/04;C12P13/04 | 主分类号 | C12N9/88 |
| 代理机构 | McDermott Will & Emery LLP | 代理人 | McDermott Will & Emery LLP |
| 主权项 | 1. A method of producing a diaminoalkylene-N,N′-disuccinate, the method comprising: reacting fumaric acid and a diamine in the presence of a host transformant or transductant comprising a recombinant expression vector comprising a gene encoding a protein having the amino acid sequence of SEQ ID NO: 1 or having an amino acid sequence derived from the amino acid sequence of SEQ ID NO: 1 by substitution of one to ten amino acid residues, wherein the protein has ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity; and collecting the diaminoalkylene-N,N′-disuccinate resulting from the reaction products, wherein the protein has ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity that is heat resistant. | ||
| 地址 | Tokyo JP | ||