| 摘要 |
One aspect as reported herein is a method for detecting a rat antibody in a serum or plasma sample (obtained) from a mouse comprising the steps of a) providing the sample to be analyzed, b) incubating said serum or plasma sample with an antibody that specifically binds to rat IgG and that does not specifically bind to mouse IgG, wherein the antibody is i) a mixture of an antibody binding to rat kappa light chain and an antibody binding to rat lambda light chain, or ii) a mixture of an antibody binding to rat IgG1 with an avidity of 4.1 x 1010 M-1 or more, an antibody binding to rat IgG2a with an avidity of 8.6 x 109 M-1 or more, an antibody binding to rat IgG2b with an avidity of 6.4 x 1010 M-1or more and an antibody binding to rat IgG2c with an avidity of 9.5 x 1010 M-1 or more, c) optionally incubating said sample with a reagent appropriate for the selective detection of total, active or antigen-bound rat antibody, and d) correlating the complex formed in (b) or (c) to the concentration of the rat antibody in the sample. |
