| 发明名称 | Method and kit for rapid isolation of human Foxp3+ Treg cells | ||
| 摘要 | The present invention relates to methods for isolating human forkhead box P3 (Foxp3+) CD4+ regulatory T cells (herein referred to a Foxp3+ Treg cells) from a sample containing (i) peripheral blood mononuclear cells (PBMCs), (ii) a lymphocyte containing fluid, or (iii) a lymphocyte containing tissue, a kit for isolating human Foxp3+ Treg cells, and the use of anti-CD49d antibody for the isolation of human Foxp3+ Treg cells. | ||
| 申请公布号 | US9213028(B2) | 申请公布日期 | 2015.12.15 |
| 申请号 | US201314089179 | 申请日期 | 2013.11.25 |
| 申请人 | MAX-DELBRUCK-CENTRUM FUR MOLEKULARE MEDIZIN (MDC) | 发明人 | Roetzschke Olaf;Falk Kirsten;Kleinewietfeld Markus |
| 分类号 | C12N5/071;G01N33/535;G01N33/569;C12N5/0783;A61K35/12 | 主分类号 | C12N5/071 |
| 代理机构 | Licata & Tyrrell P.C. | 代理人 | Licata & Tyrrell P.C. |
| 主权项 | 1. A method for isolating human Foxp3+ regulatory T cells by negative selection comprising (a) treating a sample containing (i) peripheral blood mononuclear cells, (ii) a lymphocyte containing fluid, or (iii) a lymphocyte containing tissue with antibodies, wherein said antibodies consist of an anti-CD49d antibody and an anti-CD127 antibody; and (b) depleting the sample of CD49+ and CD127+ cells via the anti-CD49d antibody and anti-CD127 antibody to obtain an enriched population of label- and antibody-free Foxp3+ regulatory T cells. | ||
| 地址 | Berlin DE | ||