摘要 |
<p>A method for determining the flux of methane newly generated through manipulation of a sub-surface environ-ment. The method comprises: (a) measuring a baseline isotope ratio (R rm) in methane pre-existing in the reservoir; (b) manipulat-ing the reservoir to facilitate biogenic production of new methane in the reservoir; (c) injecting a substance labeled with the iso-tope into the reservoir; (d) measuring a flux (F m) and isotope ratio (R pm) of methane from the reservoir; (e) measuring an isotope ratio (R pw) of a methane precursor in a liquid or vapor sample at one or more time periods; and (f) calculating a flux (F nm) of the new methane from the reservoir according to an isotope mass balance, such as wherein .alpha. = isotope fractionation factor between the methane pre-cursor and the new methane.</p> |