CIRCOVIRUS SEQUENCES ASSOCIATED WITH PORCINE WASTING DISEASE (PWD) (PWD)

摘要

Nucleotide sequence comprising a 1759 bp genomic DNA sequence (I) of a porcine circovirus called MAP ("maladie de l'amaigrissement du porcelet"; piglet fatal wasting disease), is new. Also claimed are: (1) The nucleotide sequence of the porcine circovirus is: (a) a fragment of (I); (b) a nucleotide sequence homologous to the nucleotide sequence of (a); (c) a nucleotide sequence complementary to (I) or complementary to the nucleotide sequence of (a) or (b); (d) a nucleotide sequence capable of hybridizing under stringent conditions to the nucleotide sequence of (a), (b) or (c); (e) a nucleotide sequence comprising (I) or the nucleotide sequences of (a), (b), (c) or (d); (f) a modified nucleotide sequence comprising the nucleotide sequence of (a), (b), (c), (d) or (e). (2) polypeptide encoded by the nucleotide sequence of (1); (3) cloning and/or expression vectors containing the nucleotide sequences; (4) viral or pseudoviral particles generated from the vectors; (5) host cells transformed with the vectors or (pseudo)viral particles; (6) animals containing the transformed cells; (7) synthetic polypeptides obtained using the amino acid sequence of a polypeptide as in (1); (8) hybrid polypeptides comprising at least one sequence of a polypeptide as in (2) or (7) and a sequence of an immunogenic polypeptide; (9) nucleotide sequences encoding the hybrid polypeptides; (10) vectors containing a sequence as in (9); (11) a method for the detection of the porcine circovirus; (12) mono- or polyclonal antibodies or their fragments or chimeric antibodies capable of specifically recognising a polypeptide as in (2) or (7); and (13) compounds that are capable of binding to a polypeptide or nucleotide sequence as above or of recognising an antibody as in (12) and/or of modulating, inducing or inhibiting the expression of genes and/or of modifying the cellular replication of MAP circovirus or of inducing or inhibiting pathologies associated with MAP circovirus infection in pigs and are capable of being selected by a screening assay comprising contacting a compound with a polypeptide, nucleotide sequence or transformed cell as above or administering the compound to an animal as in (6) and determining the effect of the compound.