BIOLOGICAL METHODS FOR PREPARING A FATTY DICARBOXYLIC ACID

摘要

The technology relates in part to biological methods for producing a fatty dicarboxylic acid and engineered microorganisms capable of such production. Provided are engineered microorganisms capable of producing fatty dicarboxylic acids and products expressed by such microorganisms. Also provided are biological methods for producing fatty dicarboxylic acids.

主权项

1. A genetically modified Yarrowia lipoytica yeast comprising (i) an active, modified endogenous acyl-coA oxidase polypeptide or an active, modified endogenous acyl-coA dehydrogenase polypeptide; (ii) a genetic modification that alters a native enoyl co-A isomerase activity; and (iii) a genetic modification that alters a native dieneoyl CoA reductase (DCR) activity, wherein the yeast is capable of producing a diacid from a feedstock comprising one or more components from a vegetable oil.