| 摘要 |
A method for detecting a nucleic acid amplification reaction, said method comprising carrying out a nucleic acid amplification reaction using a primer that binds a target sequence and has a tag sequence at the 5' end which is independent of said target sequence, wherein the method is carried out in the presence of a probe that is substantially homologous to said tag sequence, and a labelling system which is able to produce a detectable signal when the probe is bound to a complementary strand; measuring a signal indicative of the presence of binding of the probe to its complement, and relating this to the occurrence or progress of an amplification reaction. Kits for carrying out the method of the invention are also described and claimed. |
