COMPOSITIONS, METHODS, SYSTEMS AND KITS FOR TARGET NUCLEIC ACID ENRICHMENT

摘要

The present invention provides methods, compositions, kits, systems and apparatus that are useful for isolating nucleic acid molecules from a sample. In particular, the methods generally relate to normalizing the concentration of target nucleic acid molecules from a sample. In one aspect, the invention relates to purifying a primer extension product from a primer extension reaction mixture. In some aspects, nucleic acid molecules obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing.

主权项

1. A method for isolating a specific amount of nucleic acid from a sample, comprising:
a) generating a population of bound target nucleic acid molecules by contacting a sample including a population of target nucleic acid molecules with a limiting number of capture oligonucleotides under conditions where at least some of the capture oligonucleotides hybridize to at least some of the target nucleic acid molecules; and b) forming a population of captured target nucleic acid molecules by capturing a substantial portion of the population of bound target nucleic acid molecules, wherein the number of captured target nucleic acid molecules is directly proportional to the limiting number of capture oligonucleotides.